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KMID : 0644320000060010001
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Journal of Korean Oriental Oncology
2000 Volume.6 No. 1 p.1 ~ p.18
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Effects of the Gamihyangsayukgunjatang on Radioprotection and Apoptosis in Small Intestines of Mice
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Baek Hyung-Hee
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Abstract
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The present study was performed in order to evaluate the effects of the gamihyangsayukgunjatang on radioprotection and apoptoss in small intestines of mice after whole bdy irradiation. Two hundred forty mice were divided into 40 groups according to the radiation dose and the gamihyangsayukgunjatang treatment. The extracts of the herbal medicines were orally administered to each group differently before and/or after irradiation. The gamihyangsayukgunjatang treated groups were divided into 3 groups. Sample Ⅰ was the group treated with the gamihyangsayukgunjatang for 3 days before the radiation, sample Ⅱ was the group treated with the gamihyangsayukgunjatang for 3 days after the radiation. Sample Ⅲ was the group treated with the gamihyangsayukgunjatang for both 3 days before and after the radiation. To analyze the crypt survival, the micradony survival assay was used according to the Withers and Elind´s method. To analyze the apptosis, the TUNEL assay was done.
The reslts obtained are a follows :
1. From the microcolony survival assay, the gamihyangsayukgunjatang treated groups showed theradioprotective effect with a statistical ignificance(p<0.05), as compared to the control group. Comparing the radioprotective effect among the 3 groups, sample Ⅲ was statistically more significant than sample Ⅰand Ⅱ (p2. The results of the TUNEL assay showed that the apoptotic index in the gamihyangsayukgunjatang treaed group was slightly decreased with no effectiveness, as compared to the control group. According to the above results, it could be suggested that the gammyangsayukgummang has a prominent protective effect in mice intestines against the radiation damage. However, the radioprotective effect does not seem to be related to inhibition of the apoptosis.
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